Isolation of phloem-specific promoter for tissue-specific expression of lectin genes in plants for insect resistance
@ Isolate and characterize phloem-specific promoters such as sucrose synthase promoter from B. juncea and Arabidopsis
@ Carry out deletion analysis of the cloned promoter sequence to identify the core-promoter sequence
@ Perform genetic transformation of B. juncea with snow drop lectin gene under the control of CaMV35S and phloem-specific promoters
@ Test putative transgenic plants in lab for tissue-specific expression of lec GNA
@ Test putative transgenic plants for resistance against aphids under containments
@ Carry out deletion analysis of the cloned promoter sequence to identify the core-promoter sequence
@ Perform genetic transformation of B. juncea with snow drop lectin gene under the control of CaMV35S and phloem-specific promoters
@ Test putative transgenic plants in lab for tissue-specific expression of lec GNA
@ Test putative transgenic plants for resistance against aphids under containments